Nano-particles are important materials which can be used in many sectors such as, physical, biological, biomedical, and pharmaceutical applications. Nanotechnology is being used in developing countries to help treat disease and prevent health issues. 

 The in-vivo and in-vitro study of synthesized nano-particles in fracture bone healing of rabbit tibia bone will be a milestone work in the field of biomedical sciences  HAp is the major mineral component of human bone also

• Annually large amount of biogenic waste has been produced from slaughter houses in Nepal
• This will help in managing various natural resources